Insulin antibody (INS)

Details for Product anti-INS Antibody No. ABIN6297594
Target
Reactivity
Cow, Human, Mouse, Pig, Rabbit
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Host
Mouse
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Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
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Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
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Options
Purpose Mouse anti-Human/Cow/Pig/Rabbit/Mouse Insulin Antibody [Sodium Azide Free]
Immunogen Recombinant INS protein was used as the immunogen for the Insulin antibody.
Specificity Cytoplasmic
Purification Recombinant INS protein was used as the immunogen for the Insulin antibody.
Target
Alternative Name Insulin (INS Antibody Abstract)
Background

Target Description: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Gene Symbol: INS

Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Application Notes Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.