Keratin 10 (KRT10) antibody

Details for Product No. ABIN6297653
Antigen
  • BCIE
  • BIE
  • CK10
  • EHK
  • K10
  • KPP
  • D130054E02Rik
  • K1C1
  • Krt-1.10
  • Krt1-10
  • Ka10
  • Ker10
  • KRT10
  • KRT25
  • KRT27
  • KRT28
  • keratin 10
  • keratin 10, type I S homeolog
  • keratin, type I cytoskeletal 25
  • KRT10
  • Krt10
  • krt10.S
  • LOC101085587
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Turtle
396
151
94
56
48
6
5
4
3
2
2
1
1
1
1
Host
Mouse
251
147
4
Clonality
Monoclonal
Conjugate
Un-conjugated
18
11
7
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
203
188
184
152
98
66
42
26
24
24
14
10
10
3
2
1
Options
Purpose Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken/Turtle Acidic Cytokeratin Antibody [Sodium Azide Free]
Immunogen Solubilized keratin extract from human stratum corneum
Specificity Cytoplasmic
Purification Solubilized keratin extract from human stratum corneum
Antigen
Alternative Name Keratin, type I cytoskeletal 10 (KRT10 Antibody Abstract)
Background

Target Description: Cytokeratins, also called keratins or simply CKs, are a family of fibrous structural proteins and a major structural component in the outer layer of human skin, as well as hair and nails.  There are 20 human epithelial keratins which can be divided into two subfamiles: acidic and basic (more recent human genone sequencing has identified an additional 20 members). The formation of cytokeratin intermediate filaments requires the pairing of at least one acidic and one basic subfamily member. Members of the same keratin subfamily share extended sequence homology while members of different subfamilies show only limited sequence homology.This antibody is specific for the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), and 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

Gene Symbol: KRT10///KPP

Gene ID 3858
UniProt P13645
Application Notes FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.