CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN6297666
Target
Reactivity
Human
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Host
Mouse
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2
Clonality
Monoclonal
Conjugate
This CD45 antibody is un-conjugated
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1
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Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
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Options
Purpose Mouse anti-Human CD45RA Antibody [Sodium Azide Free]
Immunogen Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody.
Specificity Cell surface
Purification Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody.
Target
Alternative Name Receptor-type tyrosine-protein phosphatase C (PTPRC Antibody Abstract)
Background

Target Description: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Gene Symbol: PTPRC///CD45

Gene ID 5788
UniProt P08575
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes Flow Cytometry: 0.5-1 μg/million cells
Immunofluorescence: 1-2 μg/mL
Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.