CD3 epsilon antibody

Catalog No. ABIN6297680

The Mouse Monoclonal anti-CD3 epsilon antibody has been validated for FACS, IF, Func, Act and ProA. It is suitable to detect CD3 epsilon in samples from Human and Monkey.

Quick Overview for CD3 epsilon antibody (ABIN6297680)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Activation (Act), Proliferation Assay (ProA)

Product Details anti-CD3 epsilon Antibody

Purpose: Mouse anti-Human/Monkey CD3e Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: Stimulated human leukocytes were used as the immunogen for the CD3e antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD3e antibody.

Application Details

Application Notes: Functional Activity: Induce T cell activation and proliferation (Order SAF formulation)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: T-cell surface glycoprotein CD3 epsilon chain

Background:

Target Description: Recognizes the epsilon-chain of CD3 (Workshop V, Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Gene Symbol: CD3E///T3E

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway