CD44 antibody

Catalog No. ABIN6297694

This Mouse Monoclonal antibody specifically detects CD44 in FACS, WB, IHC, IF and Func. It exhibits reactivity toward Human, Baboon and Green Monkey.

Quick Overview for CD44 antibody (ABIN6297694)

Target: CD44

Reactivity: Human, Baboon, Green Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

Product Details anti-CD44 Antibody

Purpose: Mouse anti-Human/Baboon/Green Monkey CD44 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Stimulated human leukocytes were used as the immunogen for this anti-CD44 antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for this anti-CD44 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Functional Studies: Order Ab without BSA/azide
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD44

Target: CD44

Alternative Name: CD44 antigen

Background:

Target Description: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Gene Symbol: CD44///LHR///MDU2///MDU3///MIC4

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling