CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN6297701
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
  • protein tyrosine phosphatase, receptor type C
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • PTPRC
  • ptprc
  • LOC100074271
  • Ptprc
Reactivity
Human
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Host
Mouse
1210
540
291
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2
Clonality
Monoclonal
Conjugate
This CD45 antibody is un-conjugated
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Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
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Options
Purpose Mouse anti-Human CD45RA Antibody [Sodium Azide Free]
Immunogen Stimulated human leukocytes were used as the immunogen for this CD45RA antibody.
Specificity Cell surface
Purification Stimulated human leukocytes were used as the immunogen for this CD45RA antibody.
Plasmids, Primers & others Plasmids, Primers & others CD45 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Receptor-type tyrosine-protein phosphatase C (PTPRC Antibody Abstract)
Background

Target Description: This antibody recognizes a protein of 205 kDa-220 kDa, identified as CD45RA, an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ Tcells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin Tcells. T-cells expressing CD45RO are memory Tcells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral Tcells. This CD45RA antibody is useful in the study of the subpopulation of CD4+ or CD8+ T-cells. It is especially useful in differentiating T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Gene Symbol: PTPRC///CD45

Gene ID 5788
UniProt P08575
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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