Golgi Complex antibody

Catalog No. ABIN6297712

Product Details

Target: Golgi Complex

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Purpose: Mouse anti-Human Golgi Complex Antibody [Sodium Azide Free]

Specificity: Golgi complex in cytoplasm

Purification: SU-DHL-1 large cell lymphoma cells were used as the immunogen.

Immunogen: SU-DHL-1 large cell lymphoma cells were used as the immunogen.

Application Details

Application Notes: Western Blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL,
Immunocytochemistry (Acetone/paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Golgi Complex

Background:

Target Description: Clone 371-4 antibody specifically detects the Golgi complex in human cells. It is part of panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. The antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. The 371-4 antibody is an excellent marker for human cells in xenographic model research.