Golgi Complex antibody

Details for Product No. ABIN6297712
Antigen
Reactivity
Human
68
15
15
Host
Mouse
53
15
Clonality
Monoclonal
Conjugate
Un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
52
52
49
49
42
13
9
5
1
Options
Purpose Mouse anti-Human Golgi Complex Antibody [Sodium Azide Free]
Immunogen SU-DHL-1 large cell lymphoma cells were used as the immunogen.
Specificity Golgi complex in cytoplasm
Purification SU-DHL-1 large cell lymphoma cells were used as the immunogen.
Antigen
Background

Target Description: Clone 371-4 antibody specifically detects the Golgi complex in human cells. It is part of panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. The antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. The 371-4 antibody is an excellent marker for human cells in xenographic model research.

Application Notes Western Blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL,
Immunocytochemistry (Acetone/paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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