RNMT antibody

Catalog No. ABIN6297755

Product Details anti-RNMT Antibody

Target: RNMT (RNA Guanine-7 Methyltransferase (RNMT))

Reactivity: Human, Rat, Cow, Dog, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNMT antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse anti-Human/Cow/Dog/Hamster/Rat TAG-72 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and cell surface

Purification: The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this TAG-72 antibody.

Immunogen: The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this TAG-72 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for RNMT

Target: RNMT (RNA Guanine-7 Methyltransferase (RNMT))

Alternative Name: mRNA cap guanine-N7 methyltransferase   (RNMT Products)

Background:

Target Description: This antibody recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma.

Gene Symbol: tag-72  C25A1.3

Gene ID: 182875

UniProt: Q9XVS1