CA9 antibody

Catalog No. ABIN6297757

This Mouse Monoclonal antibody specifically detects CA9 in WB, IHC, IF and FACS. It exhibits reactivity toward Human and Horse.

Quick Overview for CA9 antibody (ABIN6297757)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-CA9 Antibody

Purpose: Mouse anti-Human/Horse Carbonic Anhydrase 9 Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for the Carbonic Anhydrase 9 antibody.

Immunogen: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for the Carbonic Anhydrase 9 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: Carbonic anhydrase 9

Background:

Target Description: Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Gene Symbol: CA9///G250///MN

Gene ID: 768

UniProt: Q16790