CD63 antibody

Catalog No. ABIN6297772

This Mouse Monoclonal antibody specifically detects CD63 in FACS, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for CD63 antibody (ABIN6297772)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD63 Antibody

Purpose: Mouse anti-Human CD63 Antibody Cocktail [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: The smooth plasma membrane fraction of MeWo cells (NKI/C3) and recombinant human full-length protein (LAMP3/968) were used as the immunogen for the CD63 antibody cocktail.

Immunogen: The smooth plasma membrane fraction of MeWo cells (NKI/C3) and recombinant human full-length protein (LAMP3/968) were used as the immunogen for the CD63 antibody cocktail.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD63

Target: CD63

Alternative Name: CD63 antigen

Background:

Target Description: This mAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Gene Symbol: CD63///MLA1///TSPAN30

Gene ID: 967

UniProt: P08962