CD63 antibody (PE)

Catalog No. ABIN6297775

This Mouse Monoclonal antibody specifically detects CD63 in FACS, IF and T. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD63 antibody (PE) (ABIN6297775)

Target: CD63

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Product Details anti-CD63 Antibody (PE)

Purpose: Mouse anti-Human/Mouse CD63 Antibody [PE Conjugated]

Specificity: Cytoplasmic

Purification: The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this CD63 antibody.

Immunogen: The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this CD63 antibody.

Application Details

Application Notes: Flow Cytometry: 5 µL/test/million cells or 5 µL/test/100 µL of whole blood
Immunofluorescence: 1:50-1:100 for 30 minutes at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4) with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C. The conjugate is light-sensitive, protect from light.

Target Details for CD63

Target: CD63

Alternative Name: CD63 antigen

Background:

Target Description: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Gene Symbol: CD63///MLA1///TSPAN30

Gene ID: 967

UniProt: P08962