TNF alpha antibody (Biotin)
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- Target See all TNF alpha Antibodies
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TNF alpha antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Purpose
- Mouse anti-Human TNF alpha Biotinylated Antibody
- Immunogen
- This antibody was produced by fusion of mouse myeloma with B cells obtained from a mouse immunized with a recombinant protein immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
- Clone
- 5H4-F10-E11
- Isotype
- IgG3
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha Reactivity: Human, Rat IHC, FACS, IF, StM Host: Mouse Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha Reactivity: Mouse WB Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Mouse Monoclonal C1 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 71-234) antibodyTNF alpha Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha Reactivity: Human, Mouse, Rat, Pig, Rabbit WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated
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- Application Notes
- ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000)
- Restrictions
- For Research Use only
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- Reconstitution
- A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e. g. sodium azide). The final amount is indicated on the shipping vial.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- -20°C
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- TNF alpha (TNF alpha Products)
- Background
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Target Description: TNF alpha is an adipokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages (M1), although it can be produced by many other cell types such asCD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.
- UniProt
- P78536
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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