U2AF2 antibody (N-Term)

Catalog No. ABIN629927

The Rabbit Polyclonal anti-U2AF2 antibody has been validated for WB. It is suitable to detect U2AF2 in samples from Human and Rat.

Quick Overview for U2AF2 antibody (N-Term) (ABIN629927)

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-U2AF2 Antibody

Binding Specificity: N-Term

Specificity: U2 AF2 antibody was raised against the N terminal of U2 F2

Purification: Purified

Immunogen: U2 AF2 antibody was raised using the N terminal of U2 F2 corresponding to a region with amino acids EFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSAS

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

U2AF2 Blocking Peptide, (ABIN937787), is also available for use as a blocking control in assays to test for specificity of this U2AF2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of U0 F2 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for U2AF2

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Alternative Name: U2AF2

Background: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site.

Molecular Weight: 52 kDa (MW of target protein)