U2AF2 antibody (N-Term)

Catalog No. ABIN2778722

This Rabbit Polyclonal antibody specifically detects U2AF2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for U2AF2 antibody (N-Term) (ABIN2778722)

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-U2AF2 Antibody

Binding Specificity: N-Term

Purpose: U2AF2 Antibody - N-terminal region

Sequence: EFERQLNENK QERDKENRHR KRSHSRSRSR DRKRRSRSRD RRNRDQRSAS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against U2AF2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human U2AF2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8115104

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-U2AF2 antibody (ABIN2778722)

Sickmier, Frato, Kielkopf: "Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering." in: Acta crystallographica. Section F, Structural biology and crystallization communications, Vol. 62, Issue Pt 5, pp. 457-9, (2006) (PubMed).

Target Details for U2AF2

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Alternative Name: U2AF2

Background:

Background Information: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Gene Name: U2 small nuclear RNA auxiliary factor 2

Alternative Symbols: U2AF65

Protein Name: Splicing factor U2AF 65 kDa subunit

Molecular Weight: 52kDa

Gene ID: 11338

NCBI Accession: NP_001012496

UniProt: Q96HC5