UBE2J1 antibody

Catalog No. ABIN630349

This Rabbit Polyclonal antibody specifically detects UBE2J1 in WB and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for UBE2J1 antibody (ABIN630349)

Target: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2J1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-UBE2J1 Antibody

Purification: Purified

Immunogen: UBE2 J1 antibody was raised using a synthetic peptide corresponding to a region with amino acids METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPD

Application Details

Application Notes: WB: 2.5 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

UBE2J1 Blocking Peptide, (ABIN5616869), is also available for use as a blocking control in assays to test for specificity of this UBE2J1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of UBE0 1 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for UBE2J1

Target: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Alternative Name: UBE2J1

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Molecular Weight: 35 kDa (MW of target protein)