EIF5 antibody (N-Term)

Catalog No. ABIN631763

The Rabbit Polyclonal anti-EIF5 antibody has been validated for WB. It is suitable to detect EIF5 in samples from Human, Mouse and Rat.

Quick Overview for EIF5 antibody (N-Term) (ABIN631763)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF5 Antibody

Binding Specificity: N-Term

Specificity: EIF5 antibody was raised against the N terminal of EIF5

Purification: Affinity purified

Immunogen: EIF5 antibody was raised using the N terminal of EIF5 corresponding to a region with amino acids SVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPT

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

EIF5 Blocking Peptide, (ABIN5613351), is also available for use as a blocking control in assays to test for specificity of this EIF5 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of EIF5 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: EIF5

Background: EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex.

Molecular Weight: 49 kDa (MW of target protein)