Endonuclease G antibody (Middle Region)

Catalog No. ABIN633888

This anti-Endonuclease G antibody is a Rabbit Polyclonal antibody detecting Endonuclease G in WB. Suitable for Human, Rat and Mouse.

Quick Overview for Endonuclease G antibody (Middle Region) (ABIN633888)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Endonuclease G Antibody

Binding Specificity: Middle Region

Specificity: ENDOG antibody was raised against the middle region of ENDOG

Purification: Affinity purified

Immunogen: ENDOG antibody was raised using the middle region of ENDOG corresponding to a region with amino acids YVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGS

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

ENDOG Blocking Peptide, (ABIN936261), is also available for use as a blocking control in assays to test for specificity of this ENDOG antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of ENDOG antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: ENDOG

Background: ENDOG cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities. ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

Molecular Weight: 28 kDa (MW of target protein)

Pathways: Apoptosis