VEGF antibody (Biotin)

Catalog No. ABIN636578

The Rabbit Polyclonal anti-VEGF antibody has been validated for WB and ELISA. It is suitable to detect VEGF in samples from Mouse.

Quick Overview for VEGF antibody (Biotin) (ABIN636578)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-VEGF Antibody (Biotin)

Immunogen: VEGF antibody (biotin) was raised in rabbit using highly pure recombinant murine VEGF as the immunogen.

Application Details

Application Notes: ELISA: 0.25-1 µg/mL, Inhibition: 0.08-0.12 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS, containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Background: VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), kDaR gene product (the Mouse homolog of kDaR is the flk-1 gene product) and the flt4 gene product.

Molecular Weight: 39 kDa (predicted detection band MW)