CD147 antibody

Catalog No. ABIN638433

This anti-CD147 antibody is a Mouse Monoclonal antibody detecting CD147 in WB, FACS and Func. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD147 antibody (ABIN638433)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: MEM-M6-6

Product Details anti-CD147 Antibody

Purpose: Anti-Hu CD147 Purified Low Endotoxin

Specificity: The antibody MEM-M6/6 recognizes extracellular Ig domain D2 (membrane proximal) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells, it is not expressed by resting lymphocytes.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer).

Isotype: IgG1

Application Details

Application Notes: Functional application: The antibody MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation.
Flow cytometry: recommended dilution: 1-4 μg/mL.
Western blotting: Positive control: 293 human fibroblastoid cell line, non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD147 antibody (ABIN638433)

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147

Background: Basigin (Ok blood group),CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.,Neurothelin, Basigin, LAM6, CSF, 5F7, TCSF, BSG, TCSF, Emprin, OK blood group

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins