PDCD4 antibody (AA 1-469)

Catalog No. ABIN364343

The Mouse Monoclonal anti-PDCD4 antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect PDCD4 in samples from Human.

Quick Overview for PDCD4 antibody (AA 1-469) (ABIN364343)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: K4C1

Product Details anti-PDCD4 Antibody

Binding Specificity: AA 1-469

Purification: Protein G purified

Immunogen: Recombinant human PDCD4 (1-469aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: Name/Gene ID: PDCD4
Family: Apoptosis

Synonyms: PDCD4, H731, Nuclear antigen H731-like, Nuclear antigen H731, Protein 197/15a

Gene ID: 27250

UniProt: Q53EL6