RANKL antibody (Internal Region)

Catalog No. ABIN6391341

This anti-RANKL antibody is a Goat Polyclonal antibody detecting RANKL in ELISA, FACS and IF. Suitable for Human and Mouse.

Quick Overview for RANKL antibody (Internal Region) (ABIN6391341)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RANKL antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-RANKL Antibody

Binding Specificity: Internal Region

Purpose: TNFSF11 / OPGL

Sequence: DLAKRSKLEA QP

Specificity: This antibody is expected to recognise both reported isoforms (NP_003692.1 and NP_143026.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of MCF7 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: TNFSF11

Background: TNFSF11, tumor necrosis factor (ligand) superfamily, member 11 , ODF, OPGL, RANKL, TRANCE, hRANKL2, sOdf, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nuclear factor kappa B liga

Gene ID: 8600

NCBI Accession: NP_003692, NP_143026

Pathways: NF-kappaB Signaling