IFT74 antibody

Catalog No. ABIN6391342

This Goat Polyclonal antibody specifically detects IFT74 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for IFT74 antibody (ABIN6391342)

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT74 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-IFT74 Antibody

Purpose: CMG1 / CCDC2 / IFT74

Sequence: CKTIVDALHS TSGN

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Alternative Name: IFT74

Background: CMG1, CMG-1, CMG 1, CCDC2, Coiled-coil domain containing 2, Coiled-coil domain-containing protein 2, Capillary morphogenesis gene 1, Capillary morphogenesis protein 1, IFT74, intraflagellar transport 74 homolog, Intraflagellar transport protein 74 homolog

Gene ID: 80173

NCBI Accession: NP_001092692, NP_001092693, NP_079379

Pathways: Hedgehog Signaling, Chromatin Binding