CD31 antibody (Internal Region)

Catalog No. ABIN6391357

This Goat Polyclonal antibody specifically detects CD31 in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for CD31 antibody (Internal Region) (ABIN6391357)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD31 Antibody

Binding Specificity: Internal Region

Purpose: Pecam1 / CD31 (mouse)

Sequence: EKTTIEYEVK VH

Specificity: This antibody is expected to recognize both reported isoforms (NP_032842.2, NP_001027550.1).

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80-85 kDa band observed in Mouse and Rat Spleen lysates (calculated MW of 80 kDa according to Mouse NP_032842.2 and 76.2 kDa according to Rat NP_113779.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room tempera

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: Pecam1

Background: Pecam1, platelet/endothelial cell adhesion molecule 1, C85791, Cd31, PECAM-1, Pecam, PECAM-1/CD31, platelet endothelial cell adhesion molecule

Gene ID: 18613

NCBI Accession: NP_032842, NP_001027550

Pathways: Regulation of Actin Filament Polymerization