Sonic Hedgehog antibody (C-Term)

Catalog No. ABIN6391361

The Goat Polyclonal anti-Sonic Hedgehog antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect Sonic Hedgehog in samples from Human and Mouse.

Quick Overview for Sonic Hedgehog antibody (C-Term) (ABIN6391361)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: C-Term

Purpose: SHH

Sequence: DSEALHPLGM AVK

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Human Liver lysates (calculated MW of 49.6 kDa according to NP_000184.1 Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for t

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH

Background: SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

Gene ID: 6469

NCBI Accession: NP_000184

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development