DDX58 antibody (C-Term)

Catalog No. ABIN6391376

The Goat Polyclonal anti-DDX58 antibody has been validated for IHC, ELISA, IF and FACS. It is suitable to detect DDX58 in samples from Human.

Quick Overview for DDX58 antibody (C-Term) (ABIN6391376)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DDX58 Antibody

Binding Specificity: C-Term

Purpose: DDX58

Sequence: KDFHFEKIPF DPAE

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen, Placenta, and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: DDX58

Background: DDX58 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 58, DKFZp434J1111 , DKFZp686N19181 , FLJ13599, RIG-I, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide , DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I , OTTHUMP00000045225 , RNA helicase , RNA helicase RIG-I

Gene ID: 23586

NCBI Accession: NP_055129

Pathways: Activation of Innate immune Response, Hepatitis C