PTPN22 antibody (Internal Region)

Catalog No. ABIN6391380

The Goat Polyclonal anti-PTPN22 antibody (ABIN6391380) specifically detects PTPN22 in ELISA, IHC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for PTPN22 antibody (Internal Region) (ABIN6391380)

Target: PTPN22 (Tyrosine-Protein Phosphatase Non-Receptor Type 22 (PTPN22))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PTPN22 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PTPN22 Antibody

Binding Specificity: Internal Region

Purpose: LYP / PTPN22

Sequence: CPPNKPAESV QSNNS

Specificity: This antibody is specific for human LYP1 and will not cross-react with LYP2.

Cross-Reactivity: Chimpanzee, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine, Thyroid and Adrenal Gland. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments showed a 100 kDa band in some Daudi cell lysates at 0.5-1 μg/mL. This molecular weight is routinely observed by other sources. An additional band at 75 kDa was also observed and both bands were successfully blocked by i

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PTPN22

Target: PTPN22 (Tyrosine-Protein Phosphatase Non-Receptor Type 22 (PTPN22))

Alternative Name: PTPN22

Background: PTPN22, LYP, Lyp1, Lyp2, protein tyrosine phosphatase, non-receptor type 22 (lymphoid), protein tyrosine phosphatase homolog, lymphoid-specific protein tyrosine phosphatase

Gene ID: 26191

NCBI Accession: NP_057051, NP_036543, NP_001180360, NP_001295226