SOX2 antibody (N-Term)

Catalog No. ABIN6391384

This Goat Polyclonal antibody specifically detects SOX2 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for SOX2 antibody (N-Term) (ABIN6391384)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SOX2 Antibody

Binding Specificity: N-Term

Purpose: SOX2

Sequence: YNMMETELKP PGPQQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 40 kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3 kDa according to )NP_003097.1). This molecular weight is routinely observed by other sources. Recommended concentrati

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2

Background: SOX2, SRY (sex determining region Y)-box 2, ANOP3, MGC2413 , SRY-related HMG-box gene 2, sex-determining region Y-box 2, transcription factor SOX2

Gene ID: 6657

NCBI Accession: NP_003097

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis