ASCL1 antibody (AA 79-91)

Catalog No. ABIN6391392

This anti-ASCL1 antibody is a Goat Polyclonal antibody detecting ASCL1 in WB, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for ASCL1 antibody (AA 79-91) (ABIN6391392)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ASCL1 Antibody

Binding Specificity: AA 79-91

Purpose: ASCL1 (aa79-91)

Sequence: HKSAPKQVKR QRS

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Mouse and Rat Lung lysates (calculated MW of 24.7 kDa according to Mouse NP_032579.2 and 24.9 kDa according to Rat NP_071779) . Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1

Background: ASCL1, achaete-scute complex homolog 1 (Drosophila), ASH1, HASH1, MASH1, bHLHa46, ASH-1, achaete scute protein, achaete-scute complex-like 1, achaete-scute homolog 1, class A basic helix-loop-helix protein 46

Gene ID: 429, 17172, 64186

NCBI Accession: NP_004307

Pathways: Dopaminergic Neurogenesis