Homeobox D13 antibody (Internal Region)

Catalog No. ABIN6391406

The Goat Polyclonal anti-Homeobox D13 antibody is suitable to detect Homeobox D13 in samples from Human and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Homeobox D13 antibody (Internal Region) (ABIN6391406)

Target: Homeobox D13 (HOXD13)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Homeobox D13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Homeobox D13 Antibody

Binding Specificity: Internal Region

Purpose: HOXD13

Sequence: HGVGLQQNAL KSSPH

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

Western Blot: Approx 37 kDa band observed in Rat Brain lysates (calculated MW of 34.9 kDa according to NP_001099356.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Homeobox D13

Target: Homeobox D13 (HOXD13)

Alternative Name: HOXD13

Background: HOXD13, homeobox D13, BDE, BDSD, HOX4I, SPD, homeo box 4I, homeo box D13

Gene ID: 3239, 15433, 288154

NCBI Accession: NP_000514

Pathways: Synaptic Membrane