Notch1 antibody (AA 171-182)

Catalog No. ABIN6391411

This Goat Polyclonal antibody specifically detects Notch1 in ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Notch1 antibody (AA 171-182) (ABIN6391411)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Notch1 Antibody

Binding Specificity: AA 171-182

Purpose: NOTCH1 (aa171-182)

Sequence: HGPTCRQDVN EC

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus of U2OS cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background: NOTCH1, notch 1, TAN1, hN1, Notch homolog 1, translocation-associated, neurogenic locus notch homolog protein 1, translocation-associated notch protein TAN-1

Gene ID: 4851, 18128, 25496

NCBI Accession: NP_060087

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development