GAPDH antibody (C-Term)

Catalog No. ABIN640717

This Goat not_set antibody specifically detects GAPDH in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for GAPDH antibody (C-Term) (ABIN640717)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Goat

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Specificity: DS-PB-00846 recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36kD multifunctional protein whose main function is to catalyze the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin. ImmunohistologyThis product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH9.0 is recommended for this purpose.

Cross-Reactivity: Dog (Canine)

Characteristics: Goat Anti-Human GAPDH (C-terminus)

Purification: Purified

Immunogen: Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2).

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 0.5 mg/ml

Buffer: TRIS buffered saline pH7.3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH