Natural Killer Cells antibody

Catalog No. ABIN642458

The Mouse Monoclonal anti-Natural Killer Cells antibody is suitable to detect Natural Killer Cells in samples from Mouse. It has been validated for Func, FACS and IP.

Quick Overview for Natural Killer Cells antibody (ABIN642458)

Target: Natural Killer Cells

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Application: Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details

Purpose: Mouse Anti-Mouse NK-1.1 Ag.

Specificity: DS-MB-02011 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family.In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that this antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by DS-MB-2011 appears to be even more complex, as only some CD161b positive strains are labeled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. DS-MB-02011 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.

Immunogen: Spleen and bone marrow cells from CE mice

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry, Functional Assays, Immunoprecipitation

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 1.0 mg/ml

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Natural Killer Cells