Caspase 3 antibody (Cleaved-Asp175)

Catalog No. ABIN650861

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for IHC (p) and DB. It is suitable to detect Caspase 3 in samples from Human. There are 2+ publications available.

Quick Overview for Caspase 3 antibody (Cleaved-Asp175) (ABIN650861)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Clone: RB25833

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 149-175, Cleaved-Asp175

Predicted Reactivity: B, M, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Cleaved antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-175 amino acids from human Cleaved.

Isotype: Ig Fraction

Application Details

Application Notes: IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Caspase 3 antibody (ABIN650861)

Chen, Liu, Wei, Wu, Luo, Yuan, Luo, Chen: "The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1." in: Scientific reports, Vol. 6, pp. 32189, (2018) (PubMed).

Luo, Sun, Wei, Luo, Yang, Liu, Guo, Chen: "Functional Characterization of Long Noncoding RNA Lnc_bc060912 in Human Lung Carcinoma Cells." in: Biochemistry, Vol. 54, Issue 18, pp. 2895-902, (2015) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background: CASP3 encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Molecular Weight: 31608

Gene ID: 836

NCBI Accession: NP_004337, NP_116786

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation