NFkB antibody (pSer536)

Catalog No. ABIN650886

This anti-NFkB antibody is a Rabbit Polyclonal antibody detecting NFkB in DB. Suitable for Human.

Quick Overview for NFkB antibody (pSer536) (ABIN650886)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFkB antibody is un-conjugated

Application: Dot Blot (DB)

Clone: RB22552

Product Details anti-NFkB Antibody

Binding Specificity: pSer536

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NF kappa B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NF kappa B.

Isotype: Ig Fraction

Application Details

Application Notes: DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: NF kappa B

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Molecular Weight: 60219

Gene ID: 5970

NCBI Accession: NP_001138610, NP_001230913, NP_068810

UniProt: Q04206

Pathways: Ubiquitin Proteasome Pathway, S100 Proteins