CPSF4 antibody (AA 96-123)

Catalog No. ABIN653570

The Rabbit Polyclonal anti-CPSF4 antibody is suitable to detect CPSF4 in samples from Human. It has been validated for WB.

Quick Overview for CPSF4 antibody (AA 96-123) (ABIN653570)

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB23266

Product Details anti-CPSF4 Antibody

Binding Specificity: AA 96-123

Predicted Reactivity: B, Rat, X

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CPSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human CPSF4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CPSF4

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Alternative Name: CPSF4

Background: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

Molecular Weight: 30255

Gene ID: 10898

NCBI Accession: NP_001075028, NP_006684

UniProt: O95639