SREBF2 antibody (AA 399-427)

Catalog No. ABIN654630

This anti-SREBF2 antibody is a Rabbit Polyclonal antibody detecting SREBF2 in WB, IF and IHC (p). Suitable for Human.

Quick Overview for SREBF2 antibody (AA 399-427) (ABIN654630)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB24513

Product Details anti-SREBF2 Antibody

Binding Specificity: AA 399-427

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the Central region of human SREBF2.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBF2

Background: SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.

Molecular Weight: 123688

Gene ID: 6721

NCBI Accession: NP_004590

UniProt: Q12772

Pathways: Regulation of Lipid Metabolism by PPARalpha