DDIT3 antibody (C-Term)

Catalog No. ABIN655895

The Rabbit Polyclonal anti-DDIT3 antibody has been validated for WB, IF and FACS. It is suitable to detect DDIT3 in samples from Human and Mouse.

Quick Overview for DDIT3 antibody (C-Term) (ABIN655895)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RB13788

Product Details anti-DDIT3 Antibody

Binding Specificity: AA 120-149, C-Term

Predicted Reactivity: B, Ha

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:2000. WB: 1:1000. FC: 1:25. FC: 1:25. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: DDIT3

Background: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Molecular Weight: 19175

Gene ID: 1649

NCBI Accession: NP_001181982, NP_001181983, NP_001181984, NP_001181986, NP_004074

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis