PSMD4/ASF antibody

Catalog No. ABIN6567005

The Rabbit Polyclonal anti-PSMD4/ASF antibody has been validated for WB. It is suitable to detect PSMD4/ASF in samples from Human, Mouse and Monkey.

Quick Overview for PSMD4/ASF antibody (ABIN6567005)

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD4/ASF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMD4/ASF Antibody

Purification: Affinity purification

Immunogen: Recombinant protein of human PSMD4

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PSMD4/ASF

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Alternative Name: PSMD4

Background:

Synonyms: 26S protease subunit S5a,26S proteasome non ATPase regulatory subunit 4,26S proteasome non-ATPase regulatory subunit 4,26S proteasome regulatory subunit rpn10,26S proteasome regulatory subunit S5A,AF 1,AF,AF1,Angiocidin,Antisecretory factor 1,ASF,DS5a,MCB 1,MCB1,Multiubiquitin chain binding protein,Multiubiquitin chain-binding protein,OTTHUMP00000059963,Prosome macropain,Proteasome (prosome macropain) 26S subunit non ATPase 4,Proteasome 26S non ATPase subunit 4,Proteasome 26S subunit non ATPase 4,PSMD 4,Psmd4,PSMD4,pUB R5,pUBR5,Rpn 10,Rpn10,RPN10 homolog,S5A,S5a/antisecretory factor protein

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.

Molecular Weight:

Observed_MW: 50kDa

Calculated_MW: 28kDa/40kDa

Gene ID: 5710

UniProt: P55036

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway