SPAM1 antibody

Catalog No. ABIN6569119

The Rabbit Polyclonal anti-SPAM1 antibody (ABIN6569119) specifically detects SPAM1 in IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SPAM1 antibody (ABIN6569119)

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAM1 antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-SPAM1 Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human SPAM1 (NP_003108.2).

Isotype: IgG

Application Details

Application Notes: IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SPAM1

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Alternative Name: SPAM1

Background: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Observed_MW: 47kDa

Calculated_MW: 57kDa/58kDa

Gene ID: 6677

UniProt: P38567