Laminin beta 1 antibody (C-Term)

Catalog No. ABIN657904

This Rabbit Polyclonal antibody specifically detects Laminin beta 1 in WB and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for Laminin beta 1 antibody (C-Term) (ABIN657904)

Target: Laminin beta 1 (LAMB1) (Laminin, beta 1 (LAMB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin beta 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB33607

Product Details anti-Laminin beta 1 Antibody

Binding Specificity: AA 1691-1720, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LAMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1691-1720 amino acids from the C-terminal region of human LAMB1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: LAMB1 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

Target Details for Laminin beta 1

Target: Laminin beta 1 (LAMB1) (Laminin, beta 1 (LAMB1))

Alternative Name: LAMB1

Background: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Molecular Weight: 198038

Gene ID: 3912

NCBI Accession: NP_002282

UniProt: P07942