beta Actin antibody

Catalog No. ABIN658990

This Mouse Monoclonal antibody specifically detects beta Actin in WB, IF, IHC (p), FACS and DNA Mic. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 12+ publications.

Quick Overview for beta Actin antibody (ABIN658990)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), DNA Microscopy (DNA Mic)

Clone: 137CT26-1-1

Product Details anti-beta Actin Antibody

Predicted Reactivity: X, B, C, Hs, Pr, Pig, Sh

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.

Isotype: IgG1, Ig kappa

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:10~50. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-beta Actin antibody (ABIN658990)

Lin, Chen, Huang, Xu: "CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells." in: Mediators of inflammation, Vol. 2016, pp. 1542786, (2017) (PubMed).

Munoz, Yazdi, Tang, Rivera, Taghipour, Grossman, Boone, Tasciotti: "Localized inhibition of P2X7R at the spinal cord injury site improves neurogenic bladder dysfunction by decreasing urothelial P2X3R expression in rats." in: Life sciences, Vol. 171, pp. 60-67, (2017) (PubMed).

Vispute, Bu, Le, Cheng: "Activation of GR but not PXR by dexamethasone attenuated acetaminophen hepatotoxicities via Fgf21 induction." in: Toxicology, Vol. 378, pp. 95-106, (2017) (PubMed).

Bu, Le, Zhang, Zhang, Cheng: "Berberine-induced Inactivation of Signal Transducer and Activator of Transcription 5 Signaling Promotes Male-specific Expression of a Bile Acid Uptake Transporter." in: The Journal of biological chemistry, Vol. 292, Issue 11, pp. 4602-4613, (2017) (PubMed).

Lau, Tang, Casey, Wang: "Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms." in: Oncogene, Vol. 36, Issue 27, pp. 3934-3942, (2017) (PubMed).

Martins, Bung, Koch, Richter, Schwarzmüller, Terhardt, Kurtulmus, Niehrs, Rouhi, Lohmann, Pereira, Fröhling, Glimm, Scholl: "Stk33 is required for spermatid differentiation and male fertility in mice." in: Developmental biology, Vol. 433, Issue 1, pp. 84-93, (2017) (PubMed).

Wei, Ma, Ding, Jin, Liu, Chang, Hu, Yu: "Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder." in: Neurochemical research, Vol. 41, Issue 11, pp. 3129-3137, (2016) (PubMed).

Harada, Katsuki, Takahashi, Masuda, Yoshinaga, Adachi, Izawa, Kuwamura, Nakano, Yamaji, Inui: "Androgen receptor silences thioredoxin-interacting protein and competitively inhibits glucocorticoid receptor-mediated apoptosis in pancreatic ?-Cells." in: Journal of cellular biochemistry, Vol. 116, Issue 6, pp. 998-1006, (2015) (PubMed).

Koutakis, Miserlis, Myers, Kim, Zhu, Papoutsi, Swanson, Haynatzki, Ha, Carpenter, McComb, Johanning, Casale, Pipinos et al.: "Abnormal accumulation of desmin in gastrocnemius myofibers of patients with peripheral artery disease: associations with altered myofiber morphology and density, mitochondrial dysfunction and ..." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 63, Issue 4, pp. 256-69, (2015) (PubMed).

Vatter, Di, Donaldson, Radu, Maines, Brinton: "Functional analyses of the three simian hemorrhagic fever virus nonstructural protein 1 papain-like proteases." in: Journal of virology, Vol. 88, Issue 16, pp. 9129-40, (2014) (PubMed).

Miyazawa, Tsuji: "Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene." in: Molecular biology of the cell, Vol. 25, Issue 13, pp. 2116-27, (2014) (PubMed).

Choi, Li, Felsher: "Addiction to multiple oncogenes can be exploited to prevent the emergence of therapeutic resistance." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 32, pp. E3316-24, (2014) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Molecular Weight: 41737

Gene ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction