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MYLK antibody

The Rabbit Monoclonal anti-MYLK antibody has been validated for WB and IHC (p). It is suitable to detect MYLK in samples from Human.
Catalog No. ABIN6654097

Quick Overview for MYLK antibody (ABIN6654097)

Target

See all MYLK Antibodies
MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This MYLK antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

G-13
  • Purification

    Protein A affinity

    Immunogen

    A synthetic peptide from human Myosin light chain kinase was used as the immunogen for the MLCK antibody.

    Isotype

    IgG
  • Application Notes

    Optimal dilution of the MLCK antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL

    Restrictions

    For Research Use only
  • Buffer

    Antibody in PBS with 0.02 % sodium azide and 50 % glycerol

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store the MLCK antibody at -20°C.
  • Target

    MYLK (Myosin Light Chain Kinase (MYLK))

    Alternative Name

    MYLK / MLCK

    Background

    Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA- dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury, triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis. .

    UniProt

    Q15746
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