MYLK antibody (N-Term)

Catalog No. ABIN392495

There are 2+ publications for this product available. The Rabbit Polyclonal anti-MYLK antibody is suitable to detect MYLK in samples from Human and Mouse. It has been validated for WB.

Quick Overview for MYLK antibody (N-Term) (ABIN392495)

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYLK antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB03067

Product Details anti-MYLK Antibody

Binding Specificity: AA 923-953, N-Term

Predicted Reactivity: Rb

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-953 amino acids from the N-terminal region of human MLCK.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-MYLK antibody (ABIN392495)

Rossi, Todd, Daniels, Bazan, Belayev: "Interferon Stimulated Gene 15 upregulation precedes the development of blood brain barrier disruption and cerebral edema after traumatic brain injury in young mice." in: Journal of neurotrauma, (2015) (PubMed).

Xu, Hu, Liu, Xu, Li, Wu, Shi, Tang: "P2Y6 Receptor-Mediated Microglial Phagocytosis in Radiation-Induced Brain Injury." in: Molecular neurobiology, (2015) (PubMed).

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: MLCK

Background: MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Molecular Weight: 210715

NCBI Accession: NP_444253, NP_444254, NP_444255, NP_444256, NP_444259, NP_444260

UniProt: Q15746