CD2AP antibody (AA 253-337)

Catalog No. ABIN6654151

The Mouse Monoclonal anti-CD2AP antibody has been validated for WB, FACS and IHC (p). It is suitable to detect CD2AP in samples from Human.

Quick Overview for CD2AP antibody (AA 253-337) (ABIN6654151)

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2AP antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5F8

Product Details anti-CD2AP Antibody

Binding Specificity: AA 253-337

Purification: Protein G affinity

Immunogen: Amino acids K253-K337 from the human protein were used as the immunogen for the CD2AP antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the CD2AP antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD2AP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CD2AP

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Alternative Name: CD2AP

Background: CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

UniProt: Q9Y5K6