ALPL antibody

Catalog No. ABIN6654223

The Mouse Monoclonal anti-ALPL antibody has been validated for IHC (p), FACS and IF. It is suitable to detect ALPL in samples from Human.

Quick Overview for ALPL antibody (ABIN6654223)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: V17-1

Product Details anti-ALPL Antibody

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Alkaline Phosphatase was used as the immunogen for the ALPL antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the ALPL antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ALPL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: Alkaline Phosphatase / ALPL

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.