CD40 Ligand antibody (AA 108-261)

Catalog No. ABIN6654288

This Mouse Monoclonal antibody specifically detects CD40 Ligand in IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD40 Ligand antibody (AA 108-261) (ABIN6654288)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CD40LG-2761

Product Details anti-CD40 Ligand Antibody

Binding Specificity: AA 108-261

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: A portion of amino acids 108-261 from the human protein was used as the immunogen for the CD40 Ligand antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD40 Ligand antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD40 Ligand antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L / TRAP / CD154

Background: CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints