s100a4 antibody (AA 1-200)

Catalog No. ABIN6654447

This Rabbit Monoclonal antibody specifically detects s100a4 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for s100a4 antibody (AA 1-200) (ABIN6654447)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PS1A4-1R

Product Details anti-s100a4 Antibody

Binding Specificity: AA 1-200

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the S100A4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4 / FSP1

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [RefSeq]

Pathways: S100 Proteins