BrdU antibody

Catalog No. ABIN6654471

This Rabbit Monoclonal antibody specifically detects BrdU in IHC (p). It exhibits reactivity toward .

Quick Overview for BrdU antibody (ABIN6654471)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BRD2888R

Product Details anti-BrdU Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.

Isotype: IgG kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background: This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.