Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody

Catalog No. ABIN6654519

This Rabbit Monoclonal antibody specifically detects in WB, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (ABIN6654519)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPTBN2-2887R

Product Details

Binding Specificity: AA 356-475

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.

Isotype: IgG kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,Flow cytometry: 1-2 μg/10^6 cells in 0.1ml,Immunofluorescence: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Spectrin beta III antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Alternative Name: SPTBN2 / Spectrin beta III

Background: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis