NEFH antibody

Catalog No. ABIN6654554

This anti-NEFH antibody is a Rabbit Monoclonal antibody detecting NEFH in WB and IHC (p). Suitable for Human, Rat, Mouse, Chicken and Pig.

Quick Overview for NEFH antibody (ABIN6654554)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse, Chicken, Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NEFL-H-2324R

Product Details anti-NEFH Antibody

Purification: Purified

Purity: Protein A affinity

Immunogen: Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant Neurofilament antibody.

Isotype: IgG kappa

Application Details

Application Notes: The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the recombinant Neurofilament antibody to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament (-Heavy) / NF-H / NEFH

Background: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.